32 min read

Protein Intake Guide: How Much Protein You Really Need

Protein is the most important macronutrient for body composition, muscle recovery, and long-term health. Yet most people either undereat it or have no idea how much they actually need. This guide covers everything from daily requirements by goal, to protein quality scores, to exactly how to distribute protein across your meals for maximum muscle protein synthesis.

Key Takeaways
  • Active adults need 0.7–1 g per pound of body weight daily (1.6–2.2 g/kg) for optimal muscle retention and growth
  • Protein quality matters: DIAAS scores rank digestibility and amino acid completeness—animal proteins score highest
  • Spread protein across 4–5 meals with 25–40 g per meal to maximize muscle protein synthesis
  • The leucine threshold is 2.5–3 g per meal—this triggers the mTOR pathway for muscle building
  • High protein is safe for healthy kidneys: Multiple studies confirm no kidney damage at intakes up to 3.4 g/kg
  • Older adults need more: Anabolic resistance means 1.0–1.6 g/kg minimum after age 65
  • Plant proteins work when combined properly to cover all essential amino acids
  • Thermic effect of protein: 20-30% of protein calories are burned during digestion
  • Use our free macro calculator to get your personalized protein target in seconds

Protein Requirements by Goal

Your protein needs depend heavily on your current goal. Someone in a calorie deficit needs significantly more protein per pound than someone at maintenance, because higher protein intakes protect against muscle loss during weight loss. The International Society of Sports Nutrition position stand on protein provides the evidence base for these recommendations.

Goalg/lb Body Weightg/kg Body WeightExample (170 lb / 77 kg)Notes
Weight loss (deficit)0.9–1.12.0–2.4153–187 gHigher end preserves muscle during deficit
Maintenance0.7–0.91.6–2.0119–153 gSufficient for active adults
Muscle gain (surplus)0.8–1.01.8–2.2136–170 gSurplus + training does the heavy lifting
Endurance athlete0.6–0.81.2–1.7102–136 gHigher carb needs reduce protein percentage
Strength/power athlete0.8–1.11.8–2.4136–187 gSupports maximal recovery and hypertrophy
Senior (65+)0.5–0.71.0–1.685–119 gAnabolic resistance requires higher per-meal doses
Sedentary (RDA minimum)0.360.862 gBare minimum to prevent deficiency—not optimal

The RDA of 0.8 g/kg is a floor to prevent deficiency, not an optimal target. For anyone who exercises, a higher intake is strongly supported by research. Use our macro calculator to get your exact number based on your stats and goal.

Protein Intake by Goal (g/lb body weight)
Weight Loss
0.9-1.1 g/lb
Muscle Gain
0.8-1.0 g/lb
Maintenance
0.7-0.9 g/lb
Endurance
0.6-0.8 g/lb
Sedentary (RDA)
0.36 g/lb

Complete vs. Incomplete Proteins

Proteins are made of 20 amino acids, 9 of which are essential—meaning your body cannot make them and they must come from food. A complete protein contains all 9 essential amino acids in adequate amounts. An incomplete protein is low in one or more essential amino acids.

The 9 Essential Amino Acids

  • Leucine – The primary trigger for muscle protein synthesis (MPS). Found abundantly in whey, eggs, and beef.
  • Isoleucine – Supports energy during exercise and immune function.
  • Valine – Works with leucine and isoleucine as a branched-chain amino acid (BCAA).
  • Lysine – Critical for collagen synthesis and calcium absorption. Often the limiting amino acid in grains.
  • Methionine – Precursor to cysteine and taurine. Often the limiting amino acid in legumes.
  • Phenylalanine – Precursor to tyrosine, dopamine, and adrenaline.
  • Threonine – Important for gut health and collagen production.
  • Tryptophan – Precursor to serotonin and melatonin.
  • Histidine – Needed for histamine production and red blood cell formation.

Complete protein sources include all animal proteins (meat, fish, eggs, dairy), soy, quinoa, buckwheat, and hemp seeds. Most other plant proteins are incomplete individually but become complete when combined—for example, rice and beans together provide all essential amino acids. For more on plant-based approaches, see our vegan macro calculator guide.

Essential Amino Acid Content by Protein Source

Amino AcidFunctionBest Food SourcesRDA (mg/kg/day)
LeucinePrimary MPS trigger, mTOR activationWhey (11%), Beef (8%), Eggs (8.5%)42
IsoleucineEnergy during exercise, blood sugar regulationChicken, Fish, Eggs, Cheese19
ValineMuscle metabolism, tissue repairBeef, Pork, Dairy, Legumes24
LysineCollagen synthesis, calcium absorptionBeef, Chicken, Fish, Dairy38
MethionineAntioxidant precursor, liver supportEggs, Fish, Brazil nuts19
PhenylalanineNeurotransmitter precursorBeef, Chicken, Soybeans, Fish33
ThreonineGut health, immune functionChicken, Beef, Fish, Cottage cheese20
TryptophanSerotonin and melatonin precursorTurkey, Chicken, Oats, Nuts5
HistidineHistamine production, tissue repairBeef, Fish, Poultry, Wheat14

DIAAS Protein Quality Scores

The Digestible Indispensable Amino Acid Score (DIAAS) is the current gold standard for protein quality measurement, recommended by the Food and Agriculture Organization of the United Nations. Unlike the older PDCAAS method (which was capped at 1.0), DIAAS can score above 100, reflecting that some proteins provide amino acids in excess of requirements. Higher scores mean better digestibility and amino acid completeness.

DIAAS Protein Quality Scores
Whole Milk
143
Whey Isolate
125
Eggs
113
Chicken
108
Soy Isolate
98
Pea Protein
82
Lentils
58
Wheat
41
Protein SourceDIAAS ScoreQuality RatingLimiting Amino Acid
Whole milk143ExcellentNone
Whey protein isolate125ExcellentNone
Eggs (whole)113ExcellentNone
Chicken breast108ExcellentNone
Beef (lean)106ExcellentNone
Casein protein104ExcellentNone
Salmon104ExcellentNone
Greek yogurt100ExcellentNone
Soy protein isolate98GoodMethionine
Pork loin97GoodNone
Tofu (firm)92GoodMethionine
Pea protein isolate82GoodMethionine
Chickpeas78GoodMethionine
Quinoa74GoodLeucine
Edamame71ModerateMethionine
Black beans68ModerateMethionine
Oats63ModerateLysine
Brown rice59ModerateLysine
Lentils58ModerateMethionine
Peanut butter46LowLysine & threonine
White bread41LowLysine
Almonds40LowLysine

For practical purposes, this means that if you rely primarily on plant proteins, you should eat about 10–20% more total protein to compensate for lower digestibility and amino acid profiles. Combining plant proteins that complement each other—such as legumes (low in methionine) with grains (low in lysine)—effectively raises the combined DIAAS score.

The Thermic Effect of Protein

One of the most significant advantages of a high-protein diet is the thermic effect of food (TEF). Protein requires significantly more energy to digest and process than carbohydrates or fat, making it metabolically favorable for weight management.

Thermic Effect by Macronutrient
  • Protein: 20-30% of calories burned during digestion
  • Carbohydrates: 5-10% of calories burned during digestion
  • Fat: 0-3% of calories burned during digestion
Thermic Effect of Food (TEF) - Calories Burned in Digestion
Protein
20-30%
Carbohydrates
5-10%
Fat
0-3%

Practical implication: If you eat 200 grams of protein per day (800 calories), approximately 160-240 of those calories are burned just through digestion. This is one reason why high-protein diets consistently outperform other approaches for fat loss when calories are matched. For more on optimizing your macros for weight loss, see our weight loss macros guide.

Animal vs. Plant Protein Comparison

The debate between animal and plant protein is not about which is good or bad—it is about understanding the practical trade-offs so you can make informed choices. Both can support your goals when applied correctly. For a deep dive into the plant-based side, see our vegetarian macro guide.

FactorAnimal ProteinPlant Protein
Amino acid completenessComplete (all 9 EAAs)Most are incomplete individually
DIAAS score range97–14340–98
Leucine content8–11% of total protein5–8% of total protein
Protein densityHigh (25–30 g per serving)Moderate (8–20 g per serving)
Calorie efficiencyHigh (low cal per g protein)Lower (more carbs/fat per serving)
B12 contentNaturally presentMust be supplemented
Iron formHeme iron (high absorption)Non-heme iron (lower absorption)
Fiber contentNoneOften high in fiber
Saturated fatCan be high (red meat, dairy)Generally low
Environmental impactHigher carbon footprintLower carbon footprint
Cost per gram proteinModerate ($0.03–0.08/g)Low to moderate ($0.02–0.06/g)
Muscle-building efficacySlightly superior per gramEqual when total intake is matched
Animal Protein Advantages
  • Complete amino acid profiles
  • Higher leucine for MPS
  • Better bioavailability
  • Natural B12 and heme iron
  • More protein per calorie
Plant Protein Advantages
  • Higher fiber content
  • Lower saturated fat
  • More antioxidants
  • Lower environmental impact
  • Often more affordable

Protein Timing: Distributing Protein Across Meals

How you distribute protein throughout the day matters for muscle protein synthesis (MPS). Research from Schoenfeld & Aragon (2018) shows that spreading protein across multiple meals produces superior results compared to consuming the same total in one or two large doses.

The Leucine Threshold and mTOR Activation

Muscle protein synthesis is triggered when you consume enough leucine to activate the mTOR signaling pathway. This threshold is approximately 2.5–3 grams of leucine per meal, which translates to roughly:

  • 25–30 g of animal protein (contains ~2.5–3 g leucine)
  • 35–45 g of plant protein (lower leucine percentage requires more total protein)
  • One scoop of whey protein (~25 g protein, ~2.7 g leucine)

Optimal Protein Distribution

Research suggests that hitting the leucine threshold 4–5 times per day maximizes daily MPS. Here is what an optimal distribution looks like for different protein targets:

Daily TargetMealsPer MealSample Schedule
120 g430 g8am, 12pm, 4pm, 8pm
150 g4–530–38 g8am, 11am, 2pm, 6pm, 9pm
180 g536 g7am, 10am, 1pm, 5pm, 9pm
200 g540 g7am, 10am, 1pm, 5pm, 9pm
220 g+5–637–44 g7am, 10am, 12:30pm, 3:30pm, 6:30pm, 9:30pm

Visual: Protein Distribution Patterns

Front-Loaded (common but sub-optimal)

15g
50g Lunch
55g Dinner

Even Distribution (optimal for MPS)

30g
30g
30g
30g

5-Meal Distribution (ideal for high targets)

36g
36g
36g
36g
36g

Leucine Content by Protein Source

Since leucine is the primary trigger for muscle protein synthesis, understanding which foods provide the most leucine per serving can help you make strategic food choices, especially important for older adults and those on plant-based diets.

FoodServingProtein (g)Leucine (g)Leucine %
Whey protein isolate1 scoop (25g protein)252.7511.0%
Casein protein1 scoop (24g protein)242.4010.0%
Parmesan cheese1 oz (28g)100.989.8%
Beef (lean)4 oz (113g)262.188.4%
Chicken breast4 oz (113g)262.088.0%
Eggs (whole)3 large181.448.0%
Salmon4 oz (113g)231.848.0%
Greek yogurt1 cup (227g)201.507.5%
Soybeans (edamame)1 cup (155g)181.267.0%
Pea protein1 scoop (24g protein)241.687.0%
Lentils (cooked)1 cup (198g)181.156.4%
Black beans (cooked)1 cup (172g)150.906.0%
Hemp seeds3 tbsp (30g)100.606.0%

High-Protein Foods Master Table

Below is a comprehensive list of 30+ high-protein foods ranked by protein content per standard serving. This table also includes calories and a relative cost ranking to help you build a high-protein diet on any budget. Data sourced from the Dietary Guidelines for Americans and USDA FoodData Central.

FoodServingProtein (g)CaloriesCost Rank
Chicken breast (skinless)6 oz (170 g)53281$$
Turkey breast6 oz (170 g)51270$$
Lean ground beef (93%)6 oz (170 g)48340$$
Whey protein isolate1 scoop (30 g)27120$
Tuna (canned in water)1 can (142 g)40180$
Salmon fillet6 oz (170 g)40350$$$
Shrimp6 oz (170 g)36170$$
Pork tenderloin6 oz (170 g)45280$$
Greek yogurt (nonfat)1 cup (227 g)20100$
Cottage cheese (low-fat)1 cup (226 g)28183$
Eggs (whole)3 large18210$
Egg whites1 cup (243 g)26126$
Tofu (extra firm)1 cup (252 g)20183$
Tempeh1 cup (166 g)31320$$
Lentils (cooked)1 cup (198 g)18230$
Black beans (cooked)1 cup (172 g)15227$
Chickpeas (cooked)1 cup (164 g)15269$
Edamame (shelled)1 cup (155 g)18188$
Pea protein powder1 scoop (33 g)24130$
Milk (whole)2 cups (480 ml)16298$
Milk (skim)2 cups (480 ml)17166$
Cheddar cheese2 oz (56 g)14228$$
String cheese2 sticks14160$
Bison (ground)6 oz (170 g)46324$$$
Cod fillet6 oz (170 g)40186$$
Tilapia fillet6 oz (170 g)44218$
Deli turkey4 oz (113 g)24120$$
Beef jerky1 oz (28 g)13116$$$
Seitan3 oz (85 g)21120$$
Protein bar (average)1 bar (60 g)20210$$
Peanut butter2 tbsp (32 g)7190$
Almonds1 oz (28 g)6164$$

Cost key: $ = budget-friendly, $$ = moderate, $$$ = premium. For a complete approach to meal planning with these foods, see our guide to calculating macros.

Protein Cost Comparison

Building a high-protein diet on a budget requires knowing the cost per gram of protein for different sources. This table helps you maximize protein intake while minimizing grocery spending.

Protein SourceApprox. Price/lbProtein per lbCost per 30g ProteinBudget Rating
Eggs (whole)$3.50/dozen78g/dozen$1.35Excellent
Whey protein (bulk)$12/lb120g/lb$0.75Excellent
Chicken breast (bulk)$3.50/lb140g/lb$0.75Excellent
Canned tuna$2.00/can40g/can$1.50Good
Greek yogurt$5.00/32oz80g/container$1.88Good
Cottage cheese$4.00/16oz56g/container$2.14Good
Ground beef (93%)$6.00/lb100g/lb$1.80Moderate
Salmon (fresh)$12.00/lb100g/lb$3.60Premium
Beef steak$14.00/lb120g/lb$3.50Premium
Protein bars$2.50/bar20g/bar$3.75Expensive

Protein Supplements Comparison

Supplements should complement whole foods, not replace them. That said, they can be a convenient, cost-effective way to hit your protein targets. Here is how the major supplement types compare:

Supplement TypeProtein/ScoopAbsorption SpeedLeucine ContentBest ForCost/Serving
Whey concentrate24–26 gFast (30–60 min)~2.5 gPost-workout, general use$0.60–0.90
Whey isolate27–30 gVery fast (20–40 min)~2.8 gLactose-sensitive, lean diets$0.80–1.30
Casein (micellar)24–26 gSlow (3–7 hours)~2.0 gBefore bed, sustained release$0.80–1.10
Egg white protein23–25 gMedium (1.5–3 hours)~2.2 gDairy-free, whole-food sourced$1.00–1.50
Pea protein22–25 gMedium (2–3 hours)~1.8 gVegan, allergen-free$0.70–1.00
Rice protein22–24 gMedium (2–3 hours)~1.6 gVegan, gentle on stomach$0.60–0.90
Soy protein isolate25–27 gMedium (1.5–2.5 hours)~2.0 gVegan, complete amino acids$0.50–0.80
Pea + rice blend24–26 gMedium (2–3 hours)~2.0 gVegan, improved amino profile$0.70–1.10

For most people, whey concentrate offers the best balance of quality, taste, and cost. If you follow a plant-based diet, a pea and rice protein blend provides the most complete amino acid profile. Learn more in our vegan macro calculator guide.

Protein Absorption Rates

Different protein sources are digested and absorbed at different rates. This matters for strategic meal timing around workouts and for overnight recovery.

Protein Digestion Speed (g/hour absorbed)
Whey Isolate
8-10 g/hr
Whey Concentrate
6-8 g/hr
Eggs
3-4 g/hr
Chicken/Beef
2-3 g/hr
Casein
1-2 g/hr

How Much Protein Per Meal Can Your Body Use?

A persistent myth claims that the body can only absorb 20–30 grams of protein per meal. This is a misinterpretation of the research. Your body can digest and absorb almost all the protein you eat—what has a ceiling is the acute muscle protein synthesis (MPS) response.

Research from Schoenfeld & Aragon (2018) suggests that per-meal protein intake for maximal MPS is approximately:

  • 0.4–0.55 g per kg of body weight per meal
  • For a 170 lb (77 kg) person: 31–42 g per meal
  • Protein consumed beyond this threshold is not wasted—it is used for other functions including gluconeogenesis, thermic effect of food, and tissue repair
  • A recent 2024 study showed that even 100 g of protein in a single meal continues to stimulate MPS for up to 12 hours, though the acute peak is lower than spreading it out

The practical takeaway: spread protein across 4–5 meals to maximize MPS, but do not stress if one meal is larger or smaller than the others. Total daily intake remains the most important factor by far.

Protein and Kidney Health: Debunking the Myth

One of the most persistent nutrition myths is that high protein diets damage your kidneys. Here is what the evidence actually shows, according to research reviewed by Examine.com:

  • In healthy individuals: Multiple studies with protein intakes up to 3.4 g/kg/day for up to 2 years found no adverse effects on kidney function markers (GFR, BUN, creatinine).
  • The origin of the myth: People with pre-existing chronic kidney disease (CKD) are sometimes advised to restrict protein because their compromised kidneys have difficulty filtering nitrogen waste. This was incorrectly applied to healthy individuals.
  • Large meta-analyses: A meta-analysis published in the British Journal of Sports Medicine found no evidence that high-protein diets cause kidney damage in healthy adults.
  • The NIDDK position: The National Institute of Diabetes and Digestive and Kidney Diseases distinguishes between protein restriction for existing kidney disease and the effects of protein on healthy kidneys.

Bottom line: If you have healthy kidneys, protein intakes of 1.0–1.5 g per pound of body weight are safe. Stay well hydrated, and if you have any kidney concerns, consult your doctor and get your GFR tested.

Signs of Inadequate Protein Intake

Many people unknowingly eat too little protein, especially those who are dieting or following plant-based diets without careful planning. Watch for these signs:

  • Constant hunger and cravings: Protein is the most satiating macronutrient. Low intake leads to persistent hunger despite adequate calories.
  • Slow workout recovery: If you are consistently sore for more than 48–72 hours after training, protein may be insufficient.
  • Losing strength during a cut: Rapid strength loss during a calorie deficit often indicates protein is too low. See our macros for weight loss guide for optimal deficit strategies.
  • Hair thinning or brittle nails: Hair and nails are made of protein (keratin). The body deprioritizes them when intake is low.
  • Frequent illness: The immune system relies heavily on amino acids. Low protein impairs antibody production.
  • Fatigue and brain fog: Amino acids are precursors to neurotransmitters like dopamine and serotonin.
  • Edema (swelling): Protein maintains oncotic pressure in blood vessels. Severe deficiency causes fluid retention in tissues.
  • Loss of muscle mass: If your weight is stable but you look less toned, you may be losing muscle from insufficient protein.

Protein for Special Populations

Pregnant and Breastfeeding Women

Protein needs increase during pregnancy to support fetal growth. The NIH Office of Dietary Supplements recommends:

  • Pregnancy: 1.1 g/kg/day (about 71 g for a 140 lb woman)—an increase of about 25 g over non-pregnant recommendations
  • Breastfeeding: 1.3 g/kg/day or roughly 71 g/day
  • Focus on complete protein sources and adequate folate, iron, and calcium alongside protein

Older Adults (65+)

Aging causes anabolic resistance—muscles become less responsive to the MPS-stimulating effects of protein. To overcome this:

  • Aim for 1.0–1.6 g/kg/day (higher than the general RDA of 0.8 g/kg)
  • Consume at least 25–30 g of protein per meal to overcome the elevated leucine threshold
  • Prioritize leucine-rich sources: whey, eggs, chicken, beef, dairy
  • Combine with resistance training for the most powerful anti-sarcopenia effect
  • For more on macro adjustments by age, see our beginner's guide to counting macros

Vegans and Vegetarians

Plant-based eaters can absolutely meet protein targets with some additional planning:

  • Aim for approximately 10–20% more total protein than omnivore recommendations to account for lower digestibility
  • Combine protein sources throughout the day: legumes + grains, soy products, seitan
  • Consider a pea + rice protein blend for supplementation
  • Pay special attention to leucine intake: 3.5–4 g per meal may be needed vs. 2.5–3 g for animal protein
  • See our dedicated vegan macro calculator and vegetarian macro guide for complete protocols

Athletes and Highly Active Individuals

Athletes have elevated protein needs due to greater muscle protein turnover. The American College of Sports Medicine and ISSN provide these guidelines:

  • Endurance athletes: 1.2-1.4 g/kg (0.5-0.65 g/lb)
  • Strength/power athletes: 1.6-2.2 g/kg (0.7-1.0 g/lb)
  • During caloric restriction: Up to 2.4-3.1 g/kg to preserve lean mass
  • For sport-specific recommendations, see our macro calculator for athletes

Practical Tips for Hitting Your Protein Target

  • Prioritize protein first: When building a meal, choose your protein source first and build carbs and fats around it.
  • Prep protein in bulk: Cook large batches of chicken, ground turkey, or hard-boiled eggs on Sunday for the week.
  • Use a food scale: Eyeballing protein portions can be off by 30–50%. A $10 food scale dramatically improves accuracy.
  • Keep protein snacks accessible: Greek yogurt, string cheese, jerky, and protein bars make it easy to add 15–25 g between meals.
  • Add protein to existing meals: Stir whey into oatmeal, add egg whites to scrambles, put cottage cheese on toast.
  • Do not skip breakfast protein: Most people undereat protein at breakfast. Aim for 25–40 g at your first meal.
  • Track for two weeks: Use an app like MyFitnessPal or Cronometer to learn how much protein your usual foods contain, then adjust. For macro-counting basics, see our free macro calculator guide.
  • Pre-portion your protein: Having pre-measured servings of chicken, fish, or protein powder ready to go removes guesswork.
  • Mix protein sources: Variety ensures a complete amino acid profile and prevents food fatigue.

Sample High-Protein Day (180g Protein)

Here is what a day of eating might look like to hit 180g of protein while keeping calories moderate:

MealFoodsProteinCalories
Breakfast3 eggs + 4 egg whites scrambled, 1 slice whole wheat toast32g340
Snack1 cup Greek yogurt + 1/4 cup berries20g150
Lunch6 oz chicken breast, 1 cup rice, mixed vegetables46g480
Snack1 scoop whey protein + 1 banana27g220
Dinner6 oz salmon, large salad, 1/2 cup quinoa42g520
Evening1 cup cottage cheese + 10 almonds30g270
TOTAL197g1,980

FAQ

Active adults should aim for 0.7–1 g per pound of body weight (1.6–2.2 g/kg). People in a calorie deficit should aim for the higher end to preserve muscle. Sedentary adults need at minimum 0.36 g/lb (0.8 g/kg), but this is a deficiency-prevention floor, not an optimal target.

For healthy individuals, protein intakes up to 1.5 g per pound have been studied with no adverse effects. However, excessively high protein displaces carbs and fats that are needed for energy, training performance, and hormone production. There is a point of diminishing returns beyond about 1.0–1.1 g per pound for most people.

No. In people with healthy kidneys, high-protein diets do not cause kidney damage. This has been confirmed by multiple meta-analyses. The myth comes from the fact that people with pre-existing kidney disease are sometimes advised to limit protein, which was incorrectly generalized to healthy individuals. If you have kidney concerns, get your GFR tested by your doctor.

The best sources are high in leucine and have high DIAAS scores. Whey protein, eggs, chicken breast, lean beef, and dairy top the list. Whey has the highest leucine content at about 11%, making it ideal post-workout. However, total daily protein from any quality sources matters more than any single food choice.

Your body absorbs nearly all protein you eat. The real question is how much maximally stimulates muscle protein synthesis per meal: about 0.4–0.55 g per kg of body weight, or roughly 25–40 g per meal for most people. Protein beyond this is still used for energy and other functions—it is not wasted.

No, supplements are not required if you can hit your daily target through whole foods. However, they are a convenient, cost-effective tool for people with high protein targets. Whey concentrate is typically the best value. Supplements should complement a whole-food diet, not replace it.

When total daily protein and leucine content are matched, plant and animal proteins produce similar muscle-building results. Most individual plant proteins are lower in one or more essential amino acids, so variety is key. Combining legumes with grains, or using soy-based proteins, provides a complete amino acid profile.

Both are beneficial, and the anabolic window is wider than once believed—extending several hours in both directions. If you eat a protein-rich meal 2–3 hours before training, there is no need to rush a shake immediately after. If you train fasted, post-workout protein becomes more important. Total daily intake matters most.

Common signs include constant hunger, slow recovery from workouts, loss of muscle mass or strength, brittle hair and nails, frequent illness, fatigue, and edema. If you are losing weight but also losing significant strength, insufficient protein is likely the issue.

Yes. Adults over 65 experience anabolic resistance, meaning their muscles respond less to protein. They should aim for 1.0–1.6 g/kg/day and consume at least 25–30 g per meal. Combining higher protein intake with resistance exercise is the most effective strategy against age-related muscle loss (sarcopenia).

DIAAS (Digestible Indispensable Amino Acid Score) is the current gold standard for protein quality. It measures digestibility and amino acid completeness. Scores above 100 indicate excellent protein. Whole milk scores 143, eggs 113, and chicken 108. Most plant proteins score 40–90 individually but can be combined to improve effective scores.

The thermic effect of protein is 20-30%, meaning your body uses 20-30% of the calories from protein just for digestion and processing. This is significantly higher than carbohydrates (5-10%) and fat (0-3%). This makes high-protein diets inherently more metabolically favorable for weight management.

Protein is the most filling macronutrient. It reduces levels of the hunger hormone ghrelin while boosting peptide YY, which promotes fullness. Research shows increasing protein from 15% to 30% of calories can reduce overall intake by 400+ calories per day without conscious restriction.

The leucine threshold is the minimum leucine needed to maximally stimulate muscle protein synthesis (MPS). This threshold is approximately 2.5-3 grams per meal, which corresponds to roughly 25-40 grams of protein depending on the source. It is especially important for older adults who have elevated leucine requirements.

Athletes typically need more protein than sedentary individuals. Endurance athletes should aim for 0.5-0.7g per pound (1.2-1.4g/kg), while strength and power athletes benefit from 0.7-1.0g per pound (1.6-2.2g/kg). During caloric restriction for weight class sports, protein needs increase to 1.0-1.4g per pound.

Research & References

The following studies and guidelines support the recommendations in this guide:

Calculate My Protein Target →

Related Reading